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Antioxidant Potential Analysis Of Column Chromatographic Fractions Of Euphorbia Plant Extract

BROWSE_DETAIL_CREATION_DATE: 08-03-2018

BROWSE_DETAIL_IDENTIFIER_SECTION

BROWSE_DETAIL_TYPE: Thesis

BROWSE_DETAIL_SUB_TYPE: Masters

BROWSE_DETAIL_PUBLISH_STATE: Unpublished

BROWSE_DETAIL_FORMAT: PDF Document

BROWSE_DETAIL_LANG: English

BROWSE_DETAIL_SUBJECTS: Chemical technology, Chemical engineering,

BROWSE_DETAIL_CREATORS: Elkouha, Muna Zuam Emhmed (Author),

BROWSE_DETAIL_CONTRIBUTERS: İşgör, Sultan Belgin (Advisor),

BROWSE_DETAIL_TAB_KEYWORDS

Euphorbia macroclada Boiss., polyphenolic compounds, DPPH assay,radical scavenging, antioxidant enzymes, column chromatography


BROWSE_DETAIL_TAB_ABSTRACT

The aim of this study was to assess quantity determination of Polyphenols(Total phenolic content; TPC, Total flavonoid content; TFC) and evaluateantioxidant potential of Euphorbia macroclada Boiss. leaves extract followed bytheir effect on antioxidant enzymes; Superoxide dismutase (SOD), Glutathione-Stransferase(GST) and Catalase (CAT).The methanolic leaves extract of the E.macroclada was separated with ethylacetate, n-hexane with a volume ratio of 1:10 into four fractions by using ColumnChromatography (CC) technique. Folin-Ciocalteu’s and Aluminium chloridecolorimetric method was used to analyse TPC and TFC, respectively while theantioxidant activities of column fractions were analysed by DPPH free radicalscavenging assay. The inhibitory effects of E.macroclada extract were performed bytesting the column fractions of extract on the activities of various antioxidantenzymes; SOD, GST, CAT.The results have shown that the TPC of the plant sample and its columnfractions ranged from 43.61 to 7.26 mg/ g Gallic acid equivalent while TFC rangedfrom 37.05 to 2.93 mg/ g Quercetin equivalent. The DPPH IC50 values of columnivfractions of E.macroclada extract; FII, FIII and FIV were found to be 0.2541, 0.3409and 3.42 g/l, respectively.The enzyme assays showed that the inhibitory effects of FII, FIII and FIV onGST enzyme activities were 92%, 98% and 78% respectively. Moreover, theinhibitory effects of both fraction; FII, FIII on CAT enzyme were about 99% whilefor FIV was just about 63%. Although all column fractions have strong inhibitoryeffects (especially FII, FIII) on GST and CAT enzyme activity, no inhibitory effectswere observed on SOD.


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