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Biochemical Analysis of Α- Adrenergic Receptor Inhibitors: Doxazosin and Terazosin

BROWSE_DETAIL_CREATION_DATE: 31-10-2017

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BROWSE_DETAIL_TYPE: Thesis

BROWSE_DETAIL_SUB_TYPE: Masters

BROWSE_DETAIL_PUBLISH_STATE: Unpublished

BROWSE_DETAIL_FORMAT: PDF Document

BROWSE_DETAIL_LANG: English

BROWSE_DETAIL_SUBJECTS: Chemistry, SCIENCE, Chemical engineering,

BROWSE_DETAIL_CREATORS: Salem, Amani M. M. (Author),

BROWSE_DETAIL_CONTRIBUTERS: İşgör, Sultan Belgin (Advisor),

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Alpha-Blocker Drugs, Antioxidant Enzymes, Glutathione-S-Transferase, Superoxide Dismutase, Catalase, Glutathione Peroxidase


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The objective of the study is to predict the medical purposes of alpha-blocker drugs by evaluating their effects on antioxidant defense enzymes beside to their antihypertensive actions. In order to do that, firstly Doxazosin mesylate and Terazosin hydrochloride solubility and stability were performed using UV spectrum of drugs measured at maximum wavelength of 245nm. Secondly, the effects of these drugs on the activity of antioxidant enzymes were studied for the first time with isolated or commercially available enzyme targets. In this study, the antioxidant effects of alpha-blocker drugs were analyzed by measuring the changes on the activity of these enzymes; Glutathione-S-Transferase (GST), Superoxide Dismutase (SOD), Catalase (CAT) and Glutathione Peroxidase (GPX) upon exposure to various concentrations of drugs. The results have shown that the stability of Doxazosin mesylate in phosphate buffer (pH 5.2) and Terazosin HCl in water, were not exceeding two days although it is stored at -20 C. The enzyme assays showed that the inhibitory effects of Terazosin around 90% for Catalase (CAT) with IC50 0.0002403g/L and 96% for Glutathione Peroxidase with IC50 0.00442g/L. However Terazosin have opposite behavior on Superoxide Dismutaseiv(SOD) with 70% inhibitory effect and IC50 of 0.008941g/L. The enzyme assay results showed that Doxazosin has inhibitory effect on Glutathione Peroxidase (GPx) around 85% with IC50 0.01109g/L and around 98% on Superoxide Dismutase (SOD) with IC50 0.0002177g/L., however Doxazosin have no significant effect on Catalase (CAT) enzyme. The inhibitory effects of both drugs on Glutathione-S-Transferase (GST) enzyme activity were less than 20%.


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