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The Study of Isolation and Analysis of Medicinal Potential of Stachys Cretica Lamiaceae Leaves Methanolic Extract

BROWSE_DETAIL_CREATION_DATE: 24-08-2017

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BROWSE_DETAIL_TYPE: Thesis

BROWSE_DETAIL_SUB_TYPE: Masters

BROWSE_DETAIL_PUBLISH_STATE: Unpublished

BROWSE_DETAIL_FORMAT: PDF Document

BROWSE_DETAIL_LANG: English

BROWSE_DETAIL_SUBJECTS: Chemical technology, Chemical engineering,

BROWSE_DETAIL_CREATORS: Ali, Saada S. Abdulgadar (Author),

BROWSE_DETAIL_CONTRIBUTERS: İşgör, Sultan Belgin (Advisor),

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stachys cretica plant, antioxidant enzymes, catalase, superoxide dismutase


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The advantage of studying antioxidant activity of natural products is they have high protection of body from free radicals and their effects. In this study the antioxidant effect of stachys cretica plant leaves extract was analyzed due to their total phenolic and flavonoid contents, and their effects on antioxidant systems.The chromatographic techniques such as column chromatography (CC) and thin layer chromatography (TLC) were also used. By measuring the effect of plant on the activity of the antioxidant system; glutathione-S-transferase, superoxide dismutase and catalase enzymes are used.The plant extracts were prepared by methanol extraction. The total phenolic and flavonoid contents of the fractions were compared; the fraction with highest total phenolic compounds was used for further analyses.

The inhibitory effects of leaves of plant on Glutathione-S-transferase (GST) was 100% with half maximal inhibitory concentration value IC50 of 2.639g/L andviisuperoxide dismutase (SOD) activities were 80% with IC50 of 0.7230g/L and the inhibitory effect of catalase enzyme was less than 90%with IC50 of 0.1474g/L.


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