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The Isolation And Analysis of The Medicinal Potentıal of Methanolic Extract of The Stachys Cretıca L. Flowers

BROWSE_DETAIL_CREATION_DATE: 08-08-2017

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BROWSE_DETAIL_TYPE: Thesis

BROWSE_DETAIL_SUB_TYPE: Masters

BROWSE_DETAIL_PUBLISH_STATE: Unpublished

BROWSE_DETAIL_FORMAT: PDF Document

BROWSE_DETAIL_LANG: English

BROWSE_DETAIL_SUBJECTS: Chemical technology, Chemical engineering,

BROWSE_DETAIL_CREATORS: Latresh, Malika Khalifa (Author),

BROWSE_DETAIL_CONTRIBUTERS: İşgör, Sultan Belgin (Advisor),

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Stachys Cretica plant, antioxidant enzymes, Catalase(CAT), Superoxide dismutase (SOD), Glutathione-S-transferase (GST), chromatographic techniques, medicinal plants, free radicals


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Antioxidants are vital substances which possess the ability to protect the body from damage caused by free radicals. A variety of antioxidants exits within the body and many of them are derived from dietary sources as fruits, vegetables and plants. The plants are utilized in folkloric medicine to treat many diseases.In this study, plant flowers was extracted with methanol and its total phenolic content (TPC) was measured as 25.32 mgGAE/g of extract and total flavonoid content (TFC) was measured as 29.60 mgQE/g of extract. This study utilized chromatography and was applied to separate extracts in to six fractions. The fraction utilized in enzyme assays was due to its high TFC as72.78 mgQE/g . The Glutathione-S-transferase (GST), catalase (CAT), superoxide dismutase (SOD) activities were measured by using multi-mode plate reader (Spectra MaxM2).The inhibitory effects of plant on Glutathione-S-Transferase enzyme (GST) activity was about 80% with IC50 value of 0.6001 g/L, on SOD it was about 100 % with IC50 value of 0.2909g/L. The effect of Stachys Cretica on Catalase inhibition 75% with IC50 of 0.03741g/L.


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