D.quercina mushroom, radical scavenging, antioxidant enzymes, DPPH assay, Catalase, Superoxide dismutase, Glutathione-S-transferase

Recently, there were great interest in use of mushrooms and their extracts as food supplements, since they accepted to enhance immune function, and hence promote health. They are playing important roles for treatment of numerous diseases from diabetes to various types of cancers. Today, most of the clinically effective pharmaceuticals are developed from chemical entities from mushroom in the history of medicine.In this study, total phenolic and flavonoid contents of methanol, ethanol and water extracts from D.quercina (L) Pers. was evaluated, for investigating their potential medicinal values against biological targets that are participating in the antioxidant defense system such as catalase (CAT), glutathione S-transferase (GST), and superoxide dismutase (SOD), and free radical scavenging activity. According to the results, the highest phenolic and flavonoid contents were found in the methanolicivextract of D.quercina (L) Persas 242 μg GEA /mL and 82.562μg QE/mL values, respectively. 2, 2-diphenyl-1-picrylhydrazyl (DPPH•) radical scavenging was observed with the methanol extract of D.quercina (L) Pers. with 50% inhibitory concentration value (IC50) of 0.081 g/L. The maximum GST and CAT enzyme inhibition activities were observed with methanol extract of D.quercina with 50% inhibitory concentration value (IC50) values of 0.05583g/L and 0.1458 g/L, respectively. The methanolic extract of D.quercina (L) Pers. showed less than 50% inhibitory effect on SOD enzyme activity and no IC50 value was available.