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The Effect Of Combination Of Drugs Upon K562 Cell Line Treatment

Diğer Başlık: The Effect Of Combination Of Drugs Upon K562 Cell Line Treatment

Oluşturulma Tarihi: 28-07-2019

Niteleme Bilgileri

Tür: Tez

Alt Tür: Yüksek Lisans Tezi

Yayınlanma Durumu: Yayınlanmamış

Dosya Biçimi: PDF

Dil: İngilizce

Konu(lar): Kimya mühendisliği,

Yazar(lar): Alarbi, Akhlas Salem Mohmed (Yazar),

Emeği Geçen(ler): İşgör, Sultan Belgin (Tez Danışmanı), İşgör, Yasemin Gülgün (Tez Danışmanı),


Yayın Tarihi: 28-07-2019


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Anahtar Kelimeler

Cell lines, K562, Chemotherapy, drug resistance, Genistein. SU6656, Adriamycin, Doxazocin mesylates, Antioxidant Enzymes, Glutathione-S-Transferase (GST), Superoxide Dismutase (SOD), Protein tyrosine kinase (PTK)


Özet

Nowadays, there is a very strong need for new approaches for cancer treatment. Unfortunately, treating cancer is very difficult for many reasons. The first reason is drug resistance. It is tried to approach new ways and techniques in the laboratory in order to find a permanent solution to drug resistance consequently increased the drug effectiveness. Chronic myelogenous leukemia Cell lines (K562) were used in this study to realize the effect of chemotherapeutic drugs, Doxazosin, Genistein, SU6656 and Adriamycin alone or in their different combinations on Glutathione-S-transferase (GST), Superoxide Dismutase (SOD) and Protein Tyrosine Kinase (PTK) activity. The result showed that, by administered different concentration of Genistein on K562 cells, the GST activity is directly proportional to the concentration of the drug used. While, after the K562 cells treated with different concentrations of Genistein and 7.5µM Doxazosin mesylate in combination, the GST activity was decreased gradually compared with control. On the other hand, when the cells treated with different concentration of SU6656 and 7.5 µM of Doxazosin, the highest GST activity was at the highest concentration of SU6656. By administered 7.5µM Doxazosin on K562 cells, the GST activity was decreased when it was compared with the control, however, the GST activity increased at 0.5 µM of Doxazosin. According to the experimental result, it is found that After K562 cells treated with different concentration of Genistein and 7.5µM doxazosin separately. The SOD activity increased at all concentration relative to the control. While when they administrated together doxazosin 7.5µM with variable concentration of genistein, the result showed that combined drugs increase the formazan formation 10 times much higher than the result in which each drug used alone.  While after the cells treated with 7.5 µM doxazosin in combination with variable concentration of Adriamycin, the SOD activity was increased. According to protein tyrosine kinase assay, the result showed that the activity of PTK enzyme on K562 cells decreased after treated with 7.5µM Doxazosin or by using variable concentration Genistein either alone or in their combination. while the PTK activity on K562 cells increased by using Adriamycin.  PTK activity was decreased when 7.5µM Doxazosin and variable concentration of SU6656 were administered in combination and the PTK activity was decreased when K562 cells treated with 7.5µM Doxazosin and variable concentration of Genistein in combination. As a conclusion, the results showed that Doxazosin mesylate is less toxic than the Genistein and Su6656. The activity of PTK decreases after using Doxazosin. However, Genistein can be considered as a potent inhibitor for GST enzyme activity which is an advantage to reduce drug resistance. On the other hand, according to the result, Adriamycin has the strongest toxic effect on the K562 cells.   On the other hand, Doxazosin decreases the toxicity of Genistein and SU6656 and Adriamycin when they used with them in combination.



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