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The Effect Of Combined Drug Treatment On Hl60 Cells

Diğer Başlık: The Effect Of Combined Drug Treatment On Hl60 Cells

Oluşturulma Tarihi: 24-09-2019

Niteleme Bilgileri

Tür: Tez

Alt Tür: Yüksek Lisans Tezi

Yayınlanma Durumu: Yayınlanmamış

Dosya Biçimi: PDF

Dil: İngilizce

Konu(lar): Kimya mühendisliği,

Yazar(lar): Aljadi, Hanan Salem Said (Yazar),

Emeği Geçen(ler): İşgör, Sultan Belgin (Tez Danışmanı), İşgör, Yasemin Gülgün (Tez Danışmanı),


Yayın Tarihi: 24-07-2019


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Anahtar Kelimeler

HL60; Doxazosin Mesylate;  Genistein; SU6656; Antioxidant Enzymes; Glutathione-S-Transferase; Superoxide Dismutase; Protein Tyrosine Kinase


Özet

Previous studies have demonstrated  the advantages of using the combination of drugs in the treatment of various cancers by increasing the effectiveness of one drug due to the synergistic effect  of another drug and overcome drug resistance. In this study different drugs alone and  in combinations, were used. These drugs are Doxazosin Mesylate which is  selective α1- adrenergic receptor  used as antihypertensive drug and recently has been used antitumor drug.  Genistein, is a natural  anticancer drug used to treat different type of cancers  and SU6656  which is a strong anticancer drug with high toxicity on both healthy and cancer cells which was used in this study as control for the toxicity. This study is aimed  to evaluate the effect these drugs whether alone or in combination, on cell growth of human leukemia cells (HL60) and their effect on  antioxidant enzymes; Glutathione-Stransferase (GST), Superoxide Dismutase (SOD) in addition to  Protein Tyrosine Kinase (PTK) of these cells. Firstly, human leukemia cell lines treated with different concentrations of each drug as single treatment and then subjected to different concentrations of combined drugs. The Cell viability assay was done by using the trypan blue assay  to know  the effect of each drug alone and in combination  on cell growth. The results revealed that doxazosin mesylate had less toxic effect on  the  growth of  human leukemia cells (HL60) compared to genistein and SU6656. While in combination 7.5µM of doxazosin mesylate with 0.312 µM genistein, the cytotoxic effect of anticancer drug genistein was a significantly  increased. SU6656 was more toxic on human leukemia cells. The effect of the drugs that used in this study whether alone or in combination on the activity of antioxidant enzymes GST and SOD in addition to the activity of PTK enzyme were measured and human leukemia cells used as the source of these enzymes. Enzymatic assay results have  shown that  Doxazosin mesylate and genistein  inhibit the GST activity, whether alone or in combination, while SU6656 induced the GST activity. Doxazosin mesylate and SU6656 induced  the activity of SOD enzyme while genistein alone or in combination with doxazosin mesylate inhibit the SOD  activity  of  HL60 cells. The activity of protein tyrosine kinase was induced by doxazosin mesylate whether alone or in combination with genistein. While genistein alone at higher doses induced PTK activity and at lowest doses PTK activity was inhibited. SU6656 alone and in combination with doxazosin mesylate inhibited PTK activity.



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