Bu kaydın yasal hükümlere uygun olmadığını düşünüyorsanız lütfen sayfa sonundaki Hata Bildir bağlantısını takip ederek bildirimde bulununuz. Kayıtlar ilgili üniversite yöneticileri tarafından eklenmektedir. Nadiren de olsa kayıtlarla ilgili hatalar oluşabilmektedir. MİTOS internet üzerindeki herhangi bir ödev sitesi değildir!

Antioxidant Potential Analysis Of Column Chromatographic Fractions Of Euphorbia Plant Extract

Oluşturulma Tarihi: 08-03-2018

Niteleme Bilgileri

Tür: Tez

Alt Tür: Yüksek Lisans Tezi

Yayınlanma Durumu: Yayınlanmamış

Dosya Biçimi: PDF

Dil: İngilizce

Konu(lar): Kimyasal teknoloji, Kimya mühendisliği,

Yazar(lar): Elkouha, Muna Zuam Emhmed (Yazar),

Emeği Geçen(ler): İşgör, Sultan Belgin (Danışman),

Anahtar Kelimeler

Euphorbia macroclada Boiss., polyphenolic compounds, DPPH assay,radical scavenging, antioxidant enzymes, column chromatography


Özet

The aim of this study was to assess quantity determination of Polyphenols(Total phenolic content; TPC, Total flavonoid content; TFC) and evaluateantioxidant potential of Euphorbia macroclada Boiss. leaves extract followed bytheir effect on antioxidant enzymes; Superoxide dismutase (SOD), Glutathione-Stransferase(GST) and Catalase (CAT).The methanolic leaves extract of the E.macroclada was separated with ethylacetate, n-hexane with a volume ratio of 1:10 into four fractions by using ColumnChromatography (CC) technique. Folin-Ciocalteu’s and Aluminium chloridecolorimetric method was used to analyse TPC and TFC, respectively while theantioxidant activities of column fractions were analysed by DPPH free radicalscavenging assay. The inhibitory effects of E.macroclada extract were performed bytesting the column fractions of extract on the activities of various antioxidantenzymes; SOD, GST, CAT.The results have shown that the TPC of the plant sample and its columnfractions ranged from 43.61 to 7.26 mg/ g Gallic acid equivalent while TFC rangedfrom 37.05 to 2.93 mg/ g Quercetin equivalent. The DPPH IC50 values of columnivfractions of E.macroclada extract; FII, FIII and FIV were found to be 0.2541, 0.3409and 3.42 g/l, respectively.The enzyme assays showed that the inhibitory effects of FII, FIII and FIV onGST enzyme activities were 92%, 98% and 78% respectively. Moreover, theinhibitory effects of both fraction; FII, FIII on CAT enzyme were about 99% whilefor FIV was just about 63%. Although all column fractions have strong inhibitoryeffects (especially FII, FIII) on GST and CAT enzyme activity, no inhibitory effectswere observed on SOD.


İçindekiler



Açıklamalar



Haklar



Notlar



Kaynakça


Atıf Yapanlar

Gözat Sayfasına Dön

 

Sosyal Medya ve Araçlar

İstatistikler

  • Kayıt
    • Bu ay: 6
    • Toplam: 2188
  • Online
    • Ziyaretçi: 50
    • Üye: 0
    • Toplam: 50

Detaylı İstatistikler