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The Biochemical Analysis Of Calcium Channel Blockers As Antihypertensive Drugs

Oluşturulma Tarihi: 16-08-2017

Niteleme Bilgileri

Tür: Tez

Alt Tür: Yüksek Lisans Tezi

Yayınlanma Durumu: Yayınlanmamış

Dosya Biçimi: PDF

Dil: İngilizce

Konu(lar): Kimyasal teknoloji, Kimya mühendisliği,

Yazar(lar): Maged, Mustafa Y. Saleh (Yazar),

Emeği Geçen(ler): İşgör, Sultan Belgin (Danışman),

Anahtar Kelimeler

Diltiazem Hydrochloride, Verapamil Hydrochloride, Calcium ChannelBlockers; Antioxidant Enzymes, Superoxide Dismutase, Catalase, Glutathione Peroxidase, Glutathione-S-Transferase


Özet

Many studies have proven the association between calcium channel blockers (CCBs),especially Diltiazem and Verapamil, and their relation to cancer development or theirrole on the chemotherapeutic drug efficacy. In this study, the commercially availabledrugs, diltiazem and verapamil were used to test their effects on the antioxidantenzymes, namely Superoxide Dismutase (SOD), Catalase (CAT), GlutathionePeroxidase (GPx) and Glutathione-S-Transferase (GST), with their relation to cancerdevelopment or chemotherapeutic drug resistance; to our knowledge, for the firsttime in the literature. The solubility of drugs were also performed, where certainamount of tablets extracted in different solvents, such as water, methanol andpotassium phosphate buffer (KP, 100 mM, pH 6.2). Since the higher solubility wasobserved with KP buffer, the stability tests were performed with the same solvent.For stability tests, the extracts were kept at +4ºC and the absorbance measurementswere done daily at wavelength of 236 nm for diltiazem, and 278 nm for verapamil.Both drugs were found stable for 49 days at +4ºC. Their effect on enzyme activitieswere analyzed with varying doses, and reported as percent inhibition of each enzymewith respect to control. The results showed that diltiazem inhibited SOD activityabout 100% but verapamil increased the SOD activity about 90%. The GST activityshowed that verapamil activates the enzyme about 100% but diltiazem showsiiinhibitory effect about less than 22%. For GPx, diltiazem resulted in 90% inhibitionand verapamil in 100%. On the other hand, on CAT there was no significant effectby both drugs


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