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The Isolation And Analysis of The Medicinal Potentıal of Methanolic Extract of The Stachys Cretıca L. Flowers

Oluşturulma Tarihi: 08-08-2017

Niteleme Bilgileri

Tür: Tez

Alt Tür: Yüksek Lisans Tezi

Yayınlanma Durumu: Yayınlanmamış

Dosya Biçimi: PDF

Dil: İngilizce

Konu(lar): Kimyasal teknoloji, Kimya mühendisliği,

Yazar(lar): Latresh, Malika Khalifa (Yazar),

Emeği Geçen(ler): İşgör, Sultan Belgin (Danışman),

Anahtar Kelimeler

Stachys Cretica plant, antioxidant enzymes, Catalase(CAT), Superoxide dismutase (SOD), Glutathione-S-transferase (GST), chromatographic techniques, medicinal plants, free radicals


Özet

Antioxidants are vital substances which possess the ability to protect the body from damage caused by free radicals. A variety of antioxidants exits within the body and many of them are derived from dietary sources as fruits, vegetables and plants. The plants are utilized in folkloric medicine to treat many diseases.In this study, plant flowers was extracted with methanol and its total phenolic content (TPC) was measured as 25.32 mgGAE/g of extract and total flavonoid content (TFC) was measured as 29.60 mgQE/g of extract. This study utilized chromatography and was applied to separate extracts in to six fractions. The fraction utilized in enzyme assays was due to its high TFC as72.78 mgQE/g . The Glutathione-S-transferase (GST), catalase (CAT), superoxide dismutase (SOD) activities were measured by using multi-mode plate reader (Spectra MaxM2).The inhibitory effects of plant on Glutathione-S-Transferase enzyme (GST) activity was about 80% with IC50 value of 0.6001 g/L, on SOD it was about 100 % with IC50 value of 0.2909g/L. The effect of Stachys Cretica on Catalase inhibition 75% with IC50 of 0.03741g/L.


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