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The Effect of Fomitopsis Pincola Mushroom Extract on Antioxidant Enzme Activites

Oluşturulma Tarihi: 03-03-2017

Niteleme Bilgileri

Tür: Tez

Alt Tür: Yüksek Lisans Tezi

Yayınlanma Durumu: Yayınlanmamış

Dosya Biçimi: PDF

Dil: İngilizce

Konu(lar): Kimya, Mikrobiyoloji, Kimya mühendisliği,

Yazar(lar): Elmubark, Marwa (Yazar),

Emeği Geçen(ler): İşgör, Sultan Belgin (Danışman),

Anahtar Kelimeler

Fomitopsis pinicola, radical scavenging, antioxidant enzymes, DPPHassay, Catalase, Superoxide dismutase, Glutathione Stransferase


Özet

A long time ago, mushrooms and their extracts had been widely used for medicinaland food purposes. Nutritionally, mushrooms are low in energy and fat but high inprotein, carbohydrate and dietary fiber. Mushrooms are also an important source ofbiologically active compounds with potential medicinal value. Bioactive secondarymetabolites found in mushrooms include phenolic compounds, sterols andtriterpenes. In different studies with mushrooms and isolated bioactive constituentshave many pharmacological effects such as antitumors,antioxidant, antiviral,hypocholesterolemia and hypoglycemic effects. Using of mushroom or mushroomproducts in our daily diet may provide health benefits. In this study, Fomitopsispinicola mushroom extracted with seven different methods, by using three differentsolvents; water, methanol, and ethanol. Total phenol and flavonoid contents of allthis extracts were determined to evaluate their antioxidant potency that can act assecondary defense in the biological system, beside the primary defense enzymesystems, such as catalase (CAT), superoxide dismutase, and glutathioneSivtransferase. Also the effect of this mushroom on DPPH (1,1Diphenyl2picrylhydrazyl)radical scavenging activity was examined. Since hot water boiled forone hour, and repeated for two times extracts has highest total phenolic contents(TPC) and total flavonoid contents (TFC) valves; 658.900 μg of GAE/ml of extract,and 245.800 μg of QE /ml of extract respectively. DPPH and all enzyme assays werecontinued by using this extract. This extract has ability to stabilize free radical DPPHabout 70%, with IC50 value of 0.272 g/L. also this extract inhibits CAT enzymeactivity as 50%, and on the other hand GST enzyme activity was inhibited by thesame extract as 85%. The IC50 values of CAT and GST for this extract werecalculated as 1.951×105g/L, and 0.0860.593g/L respectively. While this extract hasnot significant effect on SOD enzyme and IC50 value calculated as 0.001 g/L.


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Sosyal Medya ve Araçlar

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