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The Effect of Phellinus Torulosus Mushroom Exrtract on Anttioxidant Enyzmes

Oluşturulma Tarihi: 28-02-2017

Niteleme Bilgileri

Tür: Tez

Alt Tür: Yüksek Lisans Tezi

Yayınlanma Durumu: Yayınlanmamış

Dosya Biçimi: PDF

Dil: İngilizce

Konu(lar): Kimya, Kimya mühendisliği,

Yazar(lar): Alsamrraey, Maiser Zaid Mohye (Yazar),

Emeği Geçen(ler): İşgör, Sultan Belgin (Danışman),

Anahtar Kelimeler

Phellinus torulosus mushroom, free radical scavenging, antioxidants, DPPH assay, Catalase, Superoxide Dismutase, Glutathione-s-trasferase


Özet

In order to prevent free radical damages there has been an increasing interest in finding natural sources which can play as antioxidant. Even though several studies were done on mushrooms, Phellinus torulosus was not examined in details. In this study, the free radical scavenging capacity of the total phenolic and flavonoid contents of Phellinus torulosus mushroom extract was evaluated by DPPH and the antioxidant property was tested by catalase (CAT), superoxide dismutase (SOD) and glutathione- S-transferase (GST).In this study, four different mushroom extracts have been prepared using cold water, hot water, methanol, and ethanol. As ethanol extract resulted in the highest contents of total phenolic and flavonoid (as 625.125 μg/ml and 463.5 μg/ml respectively), the ethanol extract was used in the following steps for determining the radical scavenging capacity and antioxidant property of the mushroom.The free radical scavenging capacity of Phellinus torulosus ethanol extract was examined by using DPPH free radical. In this experiment by using quercetin and gallic acid as standards, DPPH radical scavenging capacity of mushroom extract was observed and IC50 value was calculated as 0.04353 g/l. The antioxidant property ofvPhellinus torulosus ethanol extract was determined by observing its effect on GST. The result showed that the mushroom extract inhibited the activities of GST about 60%. The effect of ethanol extract of Phellinus torulosus on GST was shown with IC50 calculated in the range of 0.1609 to 0.9076 g/l. Again in the same experiment Phellinus torulosus ethanol extract was found to have no effect on CAT and SOD enzymes with less than 15% inhibition for both enzymes.


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